no immunophenotypic abnormalities detected no immunophenotypic abnormalities detected

Integrity Aesthetic Building, 788 Banawe Avenue, Quezon City, Philippines Federal government websites often end in .gov or .mil. -MYC break-apart at 8q24, with or without IGH-BCL2 t(14;18) and BCL6 break-apart at 3q27, for suspected high grade B-cell lymphomas, based on morphologic assessment and immunophenotype (usually CD10-positive). Immunophenotype is a key parameter that is very valuable in predicting response to treatment as well as survival rates. Blood Journal v111 (8) [On-line information]. Submission of bilateral specimens is not required. Accessed January 2020. The results of this study were compared with other clinical and biological features. Examples of signs and symptoms of a blood cell cancer include: Testing may also be ordered after you have been treated for leukemia or lymphoma. Before MeSH Seiter, K. (2018 July 17, Updated). This test is appropriate for hematopoietic specimens only. Abnormal spacing of fully erupted tooth or teeth NOS; Displacement of fully erupted tooth or teeth NOS; Transposition of fully erupted . An interpretation of the immunophenotypic findings and correlation with the morphologic features will be provided by a hematopathologist for every case. Accessed April 2011. Diagnosis of malignant lymphoma - An overview. Before Nat Rev Immunol v12 (3): 191200. News-Medical, viewed 04 March 2023, https://www.news-medical.net/health/What-is-Immunophenotyping.aspx. Jevremovic D, Dronca RS, Morice WG, et al: CD5+ B-cell lymphoproliferative disorders: Beyond chronic lymphocytic leukemia and mantle cell lymphoma. The immunophenotype of adult acute myeloid leukemia: high frequency of lymphoid antigen expression and comparison of immunophenotype, French-American-British classification, and karyotypic abnormalities. ( 2006). No evidence of ATM (11q22.3) deletion. No flow cytometric abnormalities were detected in CD4-positive T-cells from 10 control patients without lymphoproliferative disorders. no immunophenotypic abnormalities detected FREE COVID TEST lansing school district spring break 2021 Book Appointment Now. Conclusion: Only 5 similar cases have been described previously. NCI CPTC Antibody Characterization Program. Imamura N, Kusunoki Y, Oda K, Abe K, Dohi H, Inada T, Kuramoto A, Kajihara H, Fujii H, Kawa K, et al. Conclusion: Only 5 similar cases have been described previously. This site needs JavaScript to work properly. Human herpesvirus-encoded kinase induces B cell lymphomas in vivo. ( 2011). However, treatment with chemotherapy may eliminate the abnormal cells, and if treatment is successful, normal white blood cells (WBCs) will replace abnormal cells. -Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm, -Acute Myeloid Leukemia: Testing Algorithm, -Acute Myeloid Leukemia: Relapsed with Previous Remission Testing Algorithm, -Acute Promyelocytic Leukemia: Guideline to Diagnosis and Follow-up, -Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow, -Acute Leukemias of Ambiguous Lineage Testing Algorithm, Acute Leukemia -- Immunophenotyping, Flow Cytometry, Chronic Lymphocytic Leukemia, Immunophenotyping, Flow Cytometry, Flow Cytometry, Leukemia Immunophenotyping, Flow Cytometry, Lymphoma Immunophenotyping, Lymphoma Immunophenotyping by Flow Cytometry, GLL Panel - Leukemia Immunophenotyping (ALWAYS order LCMS), Granular Lymphocytic Leukemia (ALWAYS order LCMS), KIR Panel - Leukemia Immunophenotyping (ALWAYS order LCMS), LGL Panel - Leukemia Immunophenotyping (ALWAYS order LCMS), NK Panel - Leukemia Immunophenotyping (ALWAYS order LCMS), B-cell ALL minimal residual disease (MRD) detection. Application of these criteria to a series of nearly 500 cases of lymphoma indicated that over 90% of B-lineage and about 80% of T-lineage neoplasms manifested immunophenotypic abnormalities that could distinguish them from benign, reactive lymphoid processes. 2. Pp 244-247. and transmitted securely. [Aggressive natural killer cell leukemia/lymphoma--possible existence of a new clinical entity originating from the third lineage of lymphoid cells]. It is important that the specimen be obtained, processed, and transported according to instructions for the other test. 1. Table 1. (accessed March 04, 2023). 2010 Sep;34(9):1235-1238. doi: 10.1016/j.leukres.2010.03.020, Immunophenotypic features by multiparameter, Shi M, Ternus JA, Ketterling RP, et al: Immunophenotypic and laboratory features of t(11;14)(q13;q32)-positive plasma cell neoplasms. American Cancer Society [On-line information]. Flow cytometry immunophenotyping may be useful in helping to diagnose, classify, treat and determine prognosis of these blood cell cancers. Study shows COVID-19 rates were likely forty-times higher than CDC estimates during BA.4/BA.5 dominant period in the U.S. Popular artificial sweetener associated with elevated risk of heart attack and stroke, study shows, Study supports the concept of atherosclerosis as a T-cell autoimmune disease targeting the arterial wall, New method can potentially catch COVID-19 infections quickly with near-perfect accuracy, Evidence that cross-reactive immunity from common human coronaviruses can influence response to SARS-CoV-2, The Effect of Intermittent Fasting on the Gut Microbiome, The Impact of Cyberbullying on Mental Health, Association between cardiovascular disease and transportation noise revealed in new research, Novel predictors of severe respiratory syncytial virus infections among infants below the age of one, Naked mRNA delivered using needle-free PYRO injection presents a safe and effective potential vaccination method, Innovative method to spot bacteria in blood, wastewater, and more, Associations between structural brain alterations and post-COVID fatigue, Reactive and neoplastic expansions of lymphocytes, Fluid suspensions (sample): flow cytometry (test method), Cells on slides (sample): immunocytochemistry (test method). Flow cytometric immunophenotyping is a valuable addition to morphology in the diagnosis of MDS in adults.7 Abnormalities detected by flow cytometry in myelomonocytic, . Correlation assay showed that t(8;21) was only present in 16 AMLM2 patients, and strongly . Hexosamine Biosynthetic Pathway Inhibition Leads to AML Cell Differentiation and Cell Death. Maturation-associated immunophenotypic abnormalities in bone marrow B-lymphocytes in myelodysplastic syndromes 7 In summary, blasts of AMoL can be. CSF cytology was negative for malignant cells. Li Y, Wei J, Mao X, Gao Q, Liu L, Cheng P, Liu L, Zhang X, Zhang K, Wang J, Zhu L, Zhou J, Zhang Y, Meng L, Sun H, Li D, Huang M, Huang W, Deng J, Zhang D. PLoS One. Accessed January 2020. We describe the clinicopathologic, cytogenetic, and molecular genetic characteristics of 14 cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with t(14;19)(q32;q13). Accessed January 2020. Unable to load your collection due to an error, Unable to load your delegates due to an error. News-Medical. Accessed April 2011. In agreement with previous studies, no immunophenotypic features (other than monocytic differentiation) predicted the presence of an 11q23 rearrangement. Most of the antigens that flow cytometry immunophenotyping detects are identified by a CD (clusters of differentiation or cluster designation) number. This study prospectively analysed the relationships between immunophenotypic and cytogenetic features of blast cells in 432 acute non-lymphoblastic leukemias (ANLL) at presentation. Application of immunophenotypic analysis in distinguishing chronic myelomonocytic leukemia from reactive monocytosis. It is not offered in every laboratory, but many larger hospitals and academic medical centers perform the testing or your sample may be sent to a reference laboratory. Flow cytometric immunophenotyping of peripheral blood, bone marrow, and body fluids is performed using the following antibodies: Triage Panel: CD3, CD10, CD16, CD19, CD34, CD45 and kappa and lambda light chains, -B-cell Panel: CD5, CD11c, CD19, CD20, CD22, CD23, CD38, CD45, CD103, CD200 and kappa and lambda light chains, -T-cell Panel: CD2, CD3, CD4, CD5, CD7, CD8, CD45, TRBC1, and gamma/delta, -Killer-cell immunoglobulin-like receptor (KIR) Panel: CD3, CD8, CD16, CD56, CD57, CD94, CD158a, CD158b, CD158e (p70), and NKG2a, -Acute Panel: CD2, CD7, CD13, CD15, CD16, CD33, CD34, CD36, CD38, CD45, CD56, CD64, CD117, and HLA-DR, -B-cell ALL, minimal residual disease (MRD) panel: CD10, CD19, CD20, CD22, CD24, CD34, CD38, CD45, CD58, and CD66c, -Myeloperoxidase (MPO)/terminal deoxynucleotidyl transferase (TdT) (MPO/TdT) Panel: cytoplasmic CD3, CD13, cytoplasmic CD22, CD34, CD45, cytoplasmic CD79a, nuclear TdT, and cytoplasmic MPO, -Plasma Cell Panel: CD19, CD38, CD45, CD138, and cytoplasmic kappa and lambda light chains, -Mast Cell Panel: CD2, CD25, CD69, CD117. A laboratory report will typically include specific results from the tests as well as an analysis of what those results mean. Understanding Laboratory Tests. Please note that medical information found and transmitted securely. Two atypical human non-Hodgkin's lymphomas (NHLs) that exhibited unusual genotypic and in situ immunophenotypic abnormalities are described. Available online at https://www.arupconsult.com/Topics/LymphomaPhenotyping.html. (Blood cells normally mature in the bone marrow and are released into circulation when they are mature or nearly mature.) Two or more immunophenotypic abnormalities were detected in 49 of 81 RCC patients (60%), and in 2 of 17 (v)SAA patients (12%). Merck Manual for Healthcare Professionals [On-line information]. Flow cytometry may be used to characterize and count types of white blood cells in the evaluation of infectious diseases, autoimmune disorders or immunodeficiencies. As part of her masters degree, she specialized in Biochemistry, with an emphasis on Microbiology, Physiology, Biotechnology, and Nutrition. Immunophenotypic abnormalities of different B-NHL subtypes are overly heterogeneous; hence, including all markers in one screening tube with kappa and lambda is difficult. Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification and prognosis of leukaemia. TdT and PAX5 were performed in five of the seven patients with ABLB detected by FC. The main advantages of IHC are the possibility to correlate antigen expression with cell morphology and tissue architecture and the ability to detect a relatively low number of neoplastic cells, such as in Hodgkin's lymphoma (HL) or T-cell-rich large B-cell lymphoma (TCRBCL). Available online at https://www.questdiagnostics.com/hcp/intguide/jsp/showintguidepage.jsp?fn=TG_Lymphoid_Neoplasms.htm. Immunophenotyping is widely used to identify and classify AML. Atypical or abnormal cells can demonstrate . Quest Diagnostics [On-line information]. Available online at https://arupconsult.com/content/acute-lymphoblastic-leukemia. Comparing cases with immunophenotypic dissimilarities to those with cytogenetic differences, no distinct patterns of association were identified. An internal organ may or may not be a little bigger or a little smaller than normal but this is insignificant and no cause for worry. Would you like email updates of new search results? Overall, del(13q14) and +12 were the most common abnormalities (39%), whereas del(11q13), del(17p13), and del(6q23) were detected only in 3, 1, and 0 cases, respectively. [On-line information]. Label specimen as spinal . with these terms and conditions. Clinical review on features and cytogenetic patterns in adult acute myeloid leukemia with lymphoid markers. Application of these criteria to a series of nearly 500 cases of lymphoma indicated that over 90% of B-lineage and about 80% of T-lineage neoplasms manifested immunophenotypic abnormalities that could distinguish them from benign, reactive lymphoid processes. Chronic active Epstein-Barr virus infection progresses to aggressive NK cell leukemia with a poor prognosis. Immunophenotyping, a common application in flow cytometry, allows multiple cell surface markers to be simultaneously characterized on a per-cell basis.Immunophenotyping can be difficult by flow cytometry, however, when only a small number of cells are available. Accessibility Accordingly, a score of 0.5, 1 or 2 was given when the value obtained for . Specimen must arrive within 96 hours of collection. Cytogenetic FISH Studies: -CCND1/IGH translocation t(11;14), to exclude mantle cell lymphoma in cases of CD5+CD23- B-cell lymphoproliferative disorder. Specimen Stability Information: Ambient/Refrigerated < or =96 hours, Slides: If possible, include 5 to 10 unstained bone marrow aspirate smears labeled with two unique identifiers. Flow lymphoma is used in the case of lymphoid neoplasms or when a lymphoid origin is suspected on the basis of cell morphology after staining. American Cancer Society. CD20 is a marker of maturity and CD34 is a marker of immaturity. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. By junio 4, 2022 masonry pilaster details junio 4, 2022 masonry pilaster details This technique helps in prognostication and is also used to differentiate between neoplastic and reactive expansions of lymphocytes. An abnormal karyotype was detected in 232 cases (54%). It can detect normal cells as well as abnormal cells whose pattern of markers are typically seen with specific types of leukemia and lymphoma. In this interview, we speak to Ceri Wiggins, a Director at AstraZeneca, about the many applications of CRISPR and its role in discovering new COPD therapies. In general, these criteria involved identification of abnormal expression or loss of antigens in B- and T-lineage populations. Am J Clin Pathol. An additional complicating factor is antigenic shift, 13 , 20 although the number of cases in which immunophenotypically aberrant blasts convert to an . Accessed January 2020. If you have a leukemia or lymphoma, routine tests such as a complete blood count (CBC) and a WBC differentialmay show an increased number of white blood cells with a predominance of one type. The synergistic proapoptotic effect of PARP-1 and HDAC inhibition in cutaneous T-cell lymphoma is mediated via Blimp-1. Chronic lymphocytic leukemia is an extremely heterogeneous disease and prognostic factors such as chromosomal abnormalities are important predictors of time to first treatment and survival. "What is Immunophenotyping?". ( 2015). Bookshelf ( 19952011). Submit only 1 of the following specimens: Preferred: Yellow top (ACD solution A or B), Acceptable: Green top (sodium heparin) or lavender top (EDTA), Slides: If possible, include 5 to 10 unstained blood smears labeled with two unique identifiers. Flow cytometry immunophenotyping may be ordered when you have an increased number of lymphocytes (or sometimes an increase in another type of white blood cell, WBC), anemia, a decreased platelet count, or immature WBCs that are not normally seen in the blood. Additionally, specific patterns of antigens are present on abnormal cells seen in leukemias and lymphomas. Multivariate analysis identified CD34 and CD9 expression as independently predictive of the presence of at least one cytogenetic abnormality (P < 10(-4) and P < 0.03, respectively). Am J Clin Pathol. -T-cell receptor gene rearrangement to examine clonality of T cells in cases showing phenotypically aberrant T-cell population. Learn more about how plasma cell neoplasms are diagnosed and treated in this expert-reviewed summary. Trisomy 12 is the second most frequent aberration detected by fluorescence in situ hybridization at the time of diagnosis (10-25%), and it confers an . 1985 Apr;65(4):974-83 First, the CD45/linear side scatter gating of flow cytometry allows the initial identification of neoplastic subpopulations for additional immunophenotypic analysis in half of ANKL cases. PMC Depending upon flow cytometry immunophenotyping results, a healthcare practitioner may determine how likely your cancer will respond to treatment and how aggressive the treatment might be. Immunophenotypically, both NHLs lacked surface Ig heavy chains. Unauthorized use of these marks is strictly prohibited. Leukemia & Lymphoma Society [On-line information]. 8600 Rockville Pike Maturation-associated immunophenotypic abnormalities in bone marrow Background: Atypical lymphocytosis is a common peripheral blood abnormality seen not only in Epstein-Barr virus (EBV)-associated acute infectious mononucleosis but also in other conditions, including other viral infections, cancer, immune . First, the CD45/linear side scatter gating of flow cytometry allows the initial identification of neoplastic subpopulations for additional immunophenotypic analysis in half of ANKL cases. What is Immunophenotyping?. Diverse immunophenotypic abnormalities were seen in patients with aHLH; the type of aberrant phenotype had no relationship to either clinical or laboratory findings, underlying/predisposing factors or to the response to treatment. A blood sample is obtained by inserting a needle into a vein. Send whole blood specimen in original tube. In the present study, we describe both quantitative and qualitative immunophenotypic abnormalities involving BM B-cells in MDS patients. Underexpression of TdT and CD79a were the most frequent abnormalities. Cancer Immunol Immunother. Accessed January 2020. Available online at https://emedicine.medscape.com/article/990113-overview. Am J Med Sci. A bone marrow sample may be collected from the hip bone by a trained health care practitioner (Bone Marrow Aspiration and Biopsy). Flow cytometry is generally used to determine cell lineage in leukemia and lymphoma. 1. In her spare time, she loves to cook up a storm in the kitchen with her super-messy baking experiments. Your questions will be answered by a laboratory scientist as part of a voluntary service provided by one of our partners, American Society for Clinical Laboratory Science. Shi M, Jevremovic D, Otteson GE, Timm MM, Olteanu H, Horna P: Single antibody detection of T-cell receptor alpha beta clonality by flow cytometry rapidly identifies mature T-cell neoplasms and monotypic small CD8-positive subsets of uncertain significance. Clipboard, Search History, and several other advanced features are temporarily unavailable. Most doctors wouldn't even bother doing a colposcopy and biopsy on a patient with ASCUS. -, Blood. Wu, A. A positive correlation was found between CD34+ and CD34 B-cell precursors (r . Bethesda, MD 20894, Web Policies More info. Ngan BY, Picker LJ, Medeiros LJ, Warnke RA. As the number of abnormal cells increases in the bone marrow, they may crowd out and inhibit the production of normal white blood cells, red blood cells, and platelets, and eventually abnormal cells may also be released into the blood. Flow cytometric immunophenotyping is an established method for the detection of occult leptomeningeal disease in patients with aggressive B-cell non-Hodgkin lymphoma, and is increasingly being used in the evaluation of patients without an established diagnosis of lymphoma who present with signs and/or symptoms referable to the central nervous Epub 2018 Aug 6. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Pagana, K. D. & Pagana, T. J. These abnormalities were related to immunophenotypic markers as detected using a consensual panel of monoclonal antibodies allowing lineage assignment and investigation of myeloid marker expression on blast cells. MDS is distinguished from other disease processes by a pattern of multiple myeloid immunophenotypic abnormalities (3-6). If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: Specimens will be initially triaged to determine which, if any, of. These tests may suggest lymphoma or leukemia, but more information is generally needed to confirm a diagnosis and to identify a specific type of leukemia or lymphoma. A pathologist, often one specializing in the study of blood diseases and/or blood cell cancers (a hematopathologist), will consider the results from the complete blood count (CBC), differential, blood smear, bone marrow findings, and flow cytometry immunophenotyping as well as other tests in order to provide a diagnostic interpretation. Please use one of the following formats to cite this article in your essay, paper or report: Cheriyedath, Susha. It depends. The .gov means its official. The t(14;19)(q32;q13) involving the IGH@ and BCL3 loci is an infrequent cytogenetic abnormality detected in B-cell malignancies. Hu X, Yang Y, Chen L, Wan Y, Sheng L, Bao Y, Zheng M. Am J Transl Res. 2019 Aug 6;9:713. doi: 10.3389/fonc.2019.00713. National Library of Medicine We use cookies to enhance your experience. In addition to reflexing flow cytometric panels, acute myeloid leukemia (AML) fluorescence in situ hybridization (FISH) testing for PML-RARA translocation t(15;17) may be added by the Mayo Clinic pathologist to exclude acute promyelocytic leukemia if there is morphologic suspicion or if blasts and promyelocytes are CD34-negative and HLA-DR-negative. SI Abnormal Reports. The immunophenotype of ANKL cells may differ from reactive NK cells in 4 respects. 2018 Aug;59(8):1913-1919. doi: 10.1080/10428194.2017.1410885, Flow Cytometry Interpretation, 2 to 8 Markers (if appropriate), Flow Cytometry Interpretation, 16 or More Markers (if appropriate), Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm, Acute Myeloid Leukemia: Testing Algorithm, Acute Myeloid Leukemia: Relapsed with Previous Remission Testing Algorithm, Acute Promyelocytic Leukemia: Guideline to Diagnosis and Follow-up, Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow, Acute Leukemias of Ambiguous Lineage Testing Algorithm, Hematopathology/Cytogenetics Test Request, Clients without access to Test Prices can contact, Prospective clients should contact their account representative. Lymphoid Neoplasms Laboratory Support of Diagnosis and Management Test Guide. Epub 2012 Sep 20. PMC FOIA Available online at https://www.cancer.org/cancer/leukemia-in-children/detection-diagnosis-staging/how-diagnosed.html. Morphologic evaluation and flow cytometric immunophenotypic analysis revealed no evidence of plasma cell neoplasm involving the BM. al. 7 In summary, blasts of AMoL can be. If the CT scan said that there are no significant abnormalities it means that nothing out of the ordinary was noted. HHS Vulnerability Disclosure, Help An abnormal karyotype was detected in 232 cases (54%). Mature B cells are normally positive for CD20 but not CD34. government site. The https:// ensures that you are connecting to the bumgarner funeral home obituaries no immunophenotypic abnormalities detected. Available online at https://www.nlm.nih.gov/medlineplus/ency/article/003518.htm. Accessed April 2011. This triage panel also determines if there is an increase in the number of T cells that aberrantly coexpress CD16, an immunophenotypic feature of T-cell granular lymphocytic leukemia. Accessed April 2011. 2018 Aug;59(8):1913-1919. doi: 10.1080/10428194.2017.1410885, 6. The testing process begins with a screening panel. Background Myeloid Sarcoma with monocytic differentiation is rare and quite likely is missed by surgical pathologists. It can be used for identifying the lineage of the cell in smears of tissues with suspected lymphoma or histocytic sarcoma. 1. Flow cytometric immunophenotyping for hematologic neoplasms. A positive correlation was found between CD34+ and CD34 B-cell precursors (r . Bethesda, MD 20894, Web Policies 8600 Rockville Pike Immunophenotyping detects the presence or absence of antigens found on the surface or interior of blood cells. Phenotypic analysis by flow cytometry of surface immunoglobulin light chains and B and T cell antigens in lymph nodes involved with non-Hodgkin's lymphoma. 2016 Aug 2;11(8):e0158827. 2015 Sep-Oct;6[5]:435-440. doi: 10.6004/jadpro.2015.6.5.4). If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. This approach generally uses less antibodies than the shotgun approach but can be more time consuming. FOIA although diagnostic criteria are well established, a no immunophenotypic myeloid abnormalities were detected in the healthy donor bone marrow aspirates or in the 10 remission bone marrow aspirates from patients with a history of nonmyeloid neoplasia table 3, as mentioned, the immunophenotypic panels used evolved during the study, and not all The translocation t(9;22)(q34;q11.2) was detected by conventional chromosomal analysis in 59 patients (91%) the Ph-positive ALL cohort. Second, unusual expression of surface antigens in ANKL cells was a prominent feature. official website and that any information you provide is encrypted Several studies have identified a relationship between AML prognosis and antigens such as CD7, CD9, CD11b, CD13, CD14, CD15, CD33, CD34, and CD56, though some other studies report conflicting results. This site needs JavaScript to work properly. Leukemia & Lymphoma Society. I got thre results today, which were "no significant abnormalities". News-Medical. Anders PM, Montgomery ND, Montgomery SA, Bhatt AP, Dittmer DP, Damania B. J Clin Invest. Using a method of analysis relying solely on immunoarchitectural features of a given case, the authors were able to define immunologic criteria capable of differentiating benign from malignant lymphoid processes independent from conventional morphologic analysis. 1985 Oct;66(4):848-58 Mosbys Diagnostic and Laboratory Test Reference 10th Edition: Mosby, Inc., Saint Louis, MO. PMC 2013 Jan;92(1):89-96. doi: 10.1007/s00277-012-1574-3. Flow cytometry immunophenotyping may be performed on blood, bone marrow, or other samples to provide this additional information. Normal granulocytes show sequential progression from promyelocytes . eCollection 2022. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. Flow cytometric immunophenotyping evaluates individual cells in suspension for the presence and absence of specific antigens (phenotype). dixie lewis car accident cause,